HPTLC Phytochemical Profiling and Simultaneous Quantification of Quercetin and Gallic in Prosopis juliflora (Sw.)

Abstract

Prosopis juliflora (Sw.) commonly known as jangli babool or vilayati babool have several bioactive compounds of therapeutic needs. The presence of various bioactive compounds and markers specifically, quercetin and gallic acid in the methanolic extract of Prosopis juliflora (fruiting aerial part) denotes their valuable pharmacological activities. Therefore, high performance thin layer chromatography (HPTLC) analysis with simultaneous quantification of quercetin and gallic acid was done for their phytochemical profiling.The phytochemical profiling and simultaneous quantification of quercetin and gallic acid of methanolic extract of Prosopis juliflora through CAMAG HPTLC analysis was determined and results were obtained in the form of chromatogram scanned at the wavelength 254nm and 366nm. Phytochemical profiling of the plant was presented in the tables showing the number of peaks, peak area, area percentage and Rf values. 14 compounds were present in sample 1 and 9 compounds were present in sample 2 denoted by the number of peaks as represented in chromatograms of test samples. Peak area and height were used to quantify quercetin and gallic acid. Quercetin was found to be 1.364% in sample 1 and 0.27% in Sample 2 while gallic acid was 0.088% in sample 1 only. Importance of this research is to rationalize the therapeutic properties of Prosopis juliflora in the indian traditional medicinal system and phytochemical profiling and simultaneous quantification of quercetin and gallic acid reveals that Prosopis juliflora have several bioactive compounds and this might be accountable for their future application in the field of pharmacognostic and pharmacological drug discovery.