Abstract
The study was aimed to validate and optimize high performance thin layer chromatography (HPTLC) method for the determination of phenolic compound hydroxycinnamic acid derivatives from the fruits of Solanum lycopersicum (tomato). The presence of caffeic acid and coumaric acid in the aqueous extracts of S. lycopersicum was observed in spectrophotometer at 200-400 nm. The phenolic functional group was recorded by FTIR analysis. In the HPTLC analysis, the pre-coated silica gel was used as the stationary phase. The solvent mixture containing toluene:ethyl acetate:formic acid:methanol (3:6:1.6:0.4) was identified as an optimum ratio were used as a mobile phase. The chromatograms of the extract was scanned by densitometer at 327 nm. The Rf values (0.67 and 0.69) and finger print data were recorded by WIN CATS software. The developed HPTLC methods for bioactive marker compounds present in the fruit were found to be simple, accurate, and economical.
Highlights
Natural product, such as plant extract, either as pure compound or as standardized extract, provides opportunities for new drug discovery
The peaks under UV visible spectrophotometer were obtained at 310 nm for standard caffeic acid and sample peak was noticed at 210 nm (Figure 1 and 2)
In High performance thin layer chromatography (HPTLC), the chromatographic conditions were based on isocratic separation with different mobile phase combinations
Summary
Natural product, such as plant extract, either as pure compound or as standardized extract, provides opportunities for new drug discovery. The premier steps to utilize the biologically active compound from the plant resources are extraction, pharmacological screening, isolation and characterization of bioactive compound, toxicological evaluation and clinical evaluation (Sasidharan et al, 2011). These active compounds are considered to be the marker or chemicals that confirms the identity of the compound. High performance thin layer chromatography (HPTLC) is a sophisticated and automated form of the thin-layer chromatography (TLC) with better and advanced separation efficiency and detection limits. It is a powerful analytical method suitable for qualitative and quantitative analytical tasks (Srivastava, 2011; Sethi, 2013)
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