Abstract
The present study was aimed to develop the high performance thin layer chromatography (HPTLC) fingerprint profile of methanol extracts of marine red alga Portieria hornemannii (Lyngbye) (Silva). A HPTLC method for the separation of the active constituents in extracts has been developed and TLC of these extracts on silica gel pre-coated aluminum plates of Merck by the automatic TLC applicator and using the solvent system n-hexane:ethyl acetate (60:40 v/v) was performed. HPTLC profiling of the extract confirm about the presence of various phytochemicals. HPTLC finger print scanned at 490 nm for methanol and ethyl acetate algal extracts revealed 13 peaks with Rf values in the range of 0.16 to 0.99 respectively. The HPTLC method for routine quality control of present species can be carried out using this method for extracts of plant and serve in qualitative, quantitative and was appropriate for standardization of the extract.
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