HPTLC Finger Print Profile of Extracts from Dried Aerial Parts of Bryophyllum Pinnatum in Different Solvents

Abstract

Introduction: Bryophyllum pinnatum Kurz. is perennial herb growing widely and used in folkoric medicine in tropical Africa, America, India, china and Australia. The divine herb has potent medicinal values and used in traditional medicine for the treatment of variety of ailments and well known for its haemostatic and wound healing properties. Methods: Chromatographic techniques were used for separation of components from different extracts of plant parts. This study was planned to develop a HPTLC fingerprint profile of drug extracts from aerial parts of Bryophyllum pinnatum in different solvents such as petroleum ether, benzene, chloroform, acetone and methanol. Results: A High Performance Thin Layer Chromatography (HPTLC) method for the separation of the active constituents in Bryophyllum pinnatum extracts has been developed and TLC of these extracts on silica gel precoated aluminum plates of Merck by automatic TLC applicator and using solvent system Chloroform: Ethanol (9.8:0.2) was performed. In the present study, HPTLC finger print of various extracts of dried aerial parts of Bryophyllum pinnatum have been carried out and the results provide referential information for standardization. Conclusion: The HPTLC method for routine quality control of present species can be carried out using this method for different extracts of plant parts and serve in qualitative, quantitative and was appropriate for standardization of the drug. The HPTLC fingerprint is also suitable for rapid and simple authentication and comparision of subtle differences among samples of identical plant resource.