Abstract

The quantitative determination by HPTLC, after iodine staining and densitometry, of three food emulsifiers phosphatidylcholine, glycerol monolaurin and sorbitan monolaurate has been fully validated. Results showed that through a temperature reduction step, the effect of iodine vapor staining could be adequately preserved for densitometry measurement. The key parameters of detection were optimized and by means of the Kubelka–Munk modeling test, the quantitative prediction of the method was verified, suggesting that this protocol would be a possible solution for the quantitation of lipids lacking a chromophore.

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