HPTLC analysis of Fumaria parviflora (Lam.) methanolic extract of whole plant

Anjali Bhargava,Anita Tilwari,Pragya Shrivastava

doi:10.1186/s43094-020-00150-x

Abstract

BackgroundFumaria parviflora (Lam.), commonly known as “fine-leaved fumitory,” is well known for its therapeutic properties in the Indian traditional medicinal system. The presence of important bioactive compounds in plants makes them pharmacologically valuable. Therefore, in the present study, the high-performance thin layer chromatography (HPTLC) analysis of Fumaria parviflora (whole plant) methanolic extract was performed for its phytochemical profiling.ResultsThe HPTLC densitometric analysis of the methanolic extract of Fumaria parviflora (whole plant) was carried out using CAMAG HPTLC system, and the results were obtained in the form of chromatograms (scanned at the wavelength of 254 nm and 366 nm) representing several peaks. The phytochemical profile of the plant was determined and presented in the tables showing the total number of peaks, peak heights, peak area, percent area, and Rf values.ConclusionThe study concluded that F. parviflora methanolic extract of the whole plant contains a rich variety of phytochemicals which might be accountable for its therapeutic value and thus justifies its traditional use in India.

Highlights

Fumaria parviflora (Lam.), commonly known as “fine-leaved fumitory,” is well known for its therapeutic properties in the Indian traditional medicinal system
Instrumentation A CAMAG high-performance thin layer chromatography (HPTLC) system equipped with LINOMAT 5 applicator fitted with 100 μl syringe, CAMAG TLC scanner, and winCATS software was used
The Retention factor (Rf) values, peak height, peak area, and percent area of the unknown substances are depicted in the tables (Tables 3, 4, 5, and 6)

Summary

Introduction

Fumaria parviflora (Lam.), commonly known as “fine-leaved fumitory,” is well known for its therapeutic properties in the Indian traditional medicinal system. Due to the presence of bioactive phytochemicals, play a very important role in human life for maintaining good health. The use of medicinal herbs in the treatment of infection is an age-old practice, and several natural products are used as phytotherapic for the treatment of many diseases [1]. There are thousands of medicinal plants known to have a long history of usage for their curative properties against various diseases and ailments [3]. Fumaria parviflora Lam., commonly known as fineleaved fumitory (in English), Shahatra, Pittapapara, or Pittapapada (in Hindi), belongs to the family Fumariaceae. Fumaria parviflora (Fumariaceae) is a pale green, diffuse, much branched annual herb widely used in Ayurvedic medicine as well as in traditional Yunani system of medicine throughout India [5]. The entire herb is traditionally used in leprosy, fever [6], and detoxification and as laxative, diuretic, and diaphoretic [7]

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Conclusion