HPTLC Analysis and Standardization of Linum usitatissimum L.

Abstract

The main objective of the study was to formulate the herbal tablets and standardizing the same for quality and purity of the formulation. In the present investigation flaxseed (Linum usitatissimum L) extract was used for preparation of tablets. From the available literatures it’s evident that there is a need for development of proper medication and dosage form for the treatment of constipation. CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner 3, and WIN CATS- 4 software were used. HPTLC finger printing of crude drug, extract and formulation of linseed seed was carried out with mobile phase, which showed different Rf values. HPTLC finger printing of crude drug, extract and formulation of linseed in the aqueous extract of L. usitatissimum showed 7 peaks by HPTLC analysis. The Rf values of standard arginine, glutamic acid and glycine were found to be 0.15, 0.62 and 0.73 respectively. The Rf values of arginine, glutamic acid and glycine of L. usitatissimum was, 0.15, 0.63 and 0.75 and mached with the standard peaks which confirmed the presence of arginine, glutamic acid and glycine in L. usitatissimum. The content of amino acid was estimated by comparing the peak area of standard and those present in the formulation. Each 10 ml of L. usitatissimum was found to contain 0.05 mg, 1.4 mg and 1.82 mg of arginine, glutamic acid and glycine respectively. Linum usitatissimum seed has a tremendous scope on further studies mainly in the area of Nutraceutical and dietary supplements