Abstract
A simple, rapid, eco-friendly and effective microextraction procedure for separation and preconcentration of nonsteroidal anti-inflammatory drugs (NSAIDs) from bovine milk samples based on in situ deep eutectic solvents formation between menthol and NSAIDs and its solidification was developed. The procedure involved alkaline hydrolysis of sample matrix, mixing aqueous sample phase with molten menthol to obtain deep eutectic solvents and analytes extraction followed by organic phase (extract) separation based on its solidification. The conditions of in situ deep eutectic solvents formation were optimized and used for the HPLC-MS/MS determination of diclofenac, flurbiprofen, ketoprofen and mefenamic acid in bovine milk samples. The developed approach allowed to exclude matrixes effects and increase enrichment factors of target analytes. The LODs, calculated from the blank tests based on 3σ, were 0.01, 0.01, 0.01 and 0.03 μg kg−1, for diclofenac, flurbiprofen, ketoprofen and mefenamic acid, respectively. The procedure was successfully applied to the analysis of real samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
