Abstract
BackgroundHyssopus cuspidatus Boriss has been used as an important ethnomedicinal plant for long to eliminate phlegm, relieve cough and as well as having antibacterial, antioxygenation, and antitumor activities. In this study, the polyphenol contents, flavonoid contents, free radical scavenging assay and animal antioxygenation property assay of ethanol extract of H. cuspidatus were measured.MethodsThis study determined the total polyphenol and flavonoid contents in H. cuspidatus by UV-VIS. Caffeic, ferulic, and rosmarinic acids were measured using HPLC-DAD. Free radical scavenging assay of H. cuspidatus was studied by colorimetric method. Animal antioxygenation property assay of H. cuspidatus was studied with mice by biochemical assay kits.ResultsThe total polyphenol and flavonoid contents of H. cuspidatus in 2017, 2018, 2019 were determined and the contents of H. cuspidatus in 2019 was the highest. In addition, rosmarinic acid was the phenolic acid with the highest content in H. cuspidatus. Compared with those of DPPH free radical, hydroxyl free radical, and superoxide anion free radical, the scavenging ability of H. cuspidatus of ABTS free radical was stronger, the average IC50 value was 0.0245 mg/mL. In animal antioxygenation property experiment, the model group was successfully established with decreased activities of SOD, CAT, and GSH-px and increased content of MDA. The ethanol extract of H. cuspidatus increased the activities of SOD, CAT, and GSH-px and reduced the content of MDA. Each group of samples and the ascorbic acid positive control group showed significant differences in the results of free radical scavenging and animal antioxygenation property experiments (P < 0.05).ConclusionsThese results suggest that H. cuspidatus exerts an antioxygenation property, which can be attributed to the contents of total polyphenol and flavonoid. Given its strong antioxygenation property, H. cuspidatus can be used as a new natural antioxidant in food preservation and disease treatment.
Highlights
Hyssopus cuspidatus Boriss has been used as an important ethnomedicinal plant for long to eliminate phlegm, relieve cough and as well as having antibacterial, antioxygenation, and antitumor activities
total polyphenol content (TPC) and total flavonoid content (TFC) determination The results showed that the TPC and TFC of H. cuspidatus was 1.34–2.06% and 3.33–4.48% (Table 1)
The results of free radical scavenging assay showed that the ethanol extract of H. cuspidatus exerted significant scavenging effects on ABTS, DPPH, hydroxyl, and superoxide anion radical
Summary
Hyssopus cuspidatus Boriss has been used as an important ethnomedicinal plant for long to eliminate phlegm, relieve cough and as well as having antibacterial, antioxygenation, and antitumor activities. Modern pharmacological research shows that H. cuspidatus can reduce or improve airway inflammation, lower blood sugar, eliminate phlegm, and relieve cough, and has many biological activities, such as antibacterial, antioxygenation, and antitumor [7]. As long as the free radical in the body are maintained in the dynamic equilibrium state of generation and elimination, the diseases caused by active oxygen and lipid peroxidation can be resisted [9, 10]. Phenolic acid, which is the main component of polyphenols in plant food, can scavenge free radical and is related to anti-cancer and antiinflammatory activities [15]. Flavonoids, which are strong antioxidants abundant in plants, can effectively scavenge oxygen free radical in the body. Epidemiology shows that flavonoids are potential adjuvant therapy for Alzheimer’s disease [16]
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