Abstract
Abstract Carbamylated peptides that were studied showed much improved resolution on C-18 reversed phase HPLC columns compared to the parent peptides. A number of dipeptides were carbamylated with ethyl-, n-propyl- or isopropyl isocyanates. The three carbamyl derivatives of each dipeptide could easily be resolved. Carbamylated dipeptides with reversed amino acid sequences were also easily separated. Methionine-enkephalin, leucine-enkephalin, Angiotensins I, II and III and substance P were carbamylated with isocyanates derived from certain carcinostatic 2-chloroethyl nitrosoureas. 1-(2-Chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU), 1-(2-chloroethyl)-3-(cis-4-hydroxycyclohexyl)-1-nitrosourea (cis-4-hydroxy-CCNU) and 1-(2-chloroethyl)-3-(trans-4-hydroxycyclohexy1)-1-nitroso urea (trans-4-hydroxy-CCNU) gave carbamylated derivatives of each peptide and each mixture of derivatives from a single parent peptide could be resolved. Conditions were found in each case whereby baseline resolution of the corresponding ...
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