Abstract
From the Figure, several sorts of amino acids are not base-line separation and require at least Rs (resolution) more than 1.5 for baseline separation. Amino acid chromatographies done by Shimazdu, Hitachi or Nihon Bunko were almost identical. They require baseline separation; otherwise reproducible determination cannot be attained. For that purpose of base-line separation, gradient elution condition must be innovated. Most innovated point is the use of column with higher theoretical plate number. So, I suppose why they do not use capillary-column with higher theoretical plate number. The above presented chromatogram is representative chromatogram, so analytical validation is further required and gradient condition and elution condition must be innovated to attain base-line separation chromatogram.
Highlights
Concerning amino acid analysis, it is so often used post column derivatization method using ninhydrin or ortho-phthal aldehyde with gradient elution
Problem is that 21 sorts of amino acids cannot always baseline separation (Figure 1)
Amino acid chromatographies done by Shimazdu, Hitachi or Nihon Bunko were almost identical
Summary
Concerning amino acid analysis, it is so often used post column derivatization method using ninhydrin or ortho-phthal aldehyde with gradient elution. HPLC Separation of Amino Acids is Appropriate? Hideharu Shintani* School of Science, Chuo University, 1-13-27, Kasuga Bunkyo 112-0003, Tokyo, Japan Problem is that 21 sorts of amino acids cannot always baseline separation (Figure 1).
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