Abstract
A reversed-phase HPLC system for the quantification of recombinant DNA insulin precursors in yeast fermentation broth is described. HPLC monitoring of the polypeptide can be achieved without interference from substrate components or proteins stemming from the host cell, thus serving to determine the optimum harvesting time. Furthermore, it is demonstrated how different amino acid links of the insulin A- and B-chains affect the chromatographic behaviour of the insulin precursor making it possible to identify each precursor by its retention on the reversed phase support.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
