HPLC- PDA and UV-VIS Spectrometry Analysis used to Fingerprint Sea Buckthorn (Hippophae rhamnoides L.) Berries Comparatively with Leaves and Seeds Extracts

Abstract

The carotenoids and chlorophylls from sea buckthorn (Hippophae rhamnoides L., ssp. Carpatica) berries, leaves and seeds were identified by HPLC –PDA and quantified by UV-VIS spectrometry. The HPLC chromatograms showed that among carotenoids, lutein (tR= 11.093 min) was the major compound followed by β carotene (tR= 25.04 min), zeaxanthin (tR= 11.51 min), small quantities of neoxanthin (tR= 6.93 min), violaxanthin (tR= 4.66 min) and traces of β cryptoxanthin (tR= 18.14 min).The identified chlorophylls were: chlorophyll a (tR= 17.55 min), chlorophyll b (tR =14.97 min) and pheophytin a (tR= 23.82 min). In accordance with the HPLC fingerprint, the chlorophyll content was: 0% in berries, 41.16 % in leaves and 16.36% in seeds. The total carotenoid concentrations were 293.59, 264.80 and 3.63 mg /kg fresh berries, leaves and seeds, respectively.