Abstract
An automatic monitoring system using HPLC was developed and applied to lactic acid fermentation on a pilot scale. The system consisted of a cross-flow filter, a dilution system, an automatic sampling system, and a high performance liquid chromatograph. The lactic acid and glucose concentrations were measured every 30 min. From these results the relationship between the specific growth rate and the specific production rate of lactic acid which we reported in a previous paper at glucose concentrations of 10, 20, and 30 g/ l was verified at a higher concentration of glucose (120 g/ l).
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