HPLC Methods for Fucoxanthin Analysis in Dietary Supplements

Abstract

Fucoxanthin (FX) is the second most prominent carotenoid in the earth's biomass. It is the primary carotenoid in some forms of brown algae. FX has been shown to reduce visceral adipose in mice and humans and is being incorporated into dietary supplements for weight loss. To examine the content of commercial FX products, we developed and validated Normal‐Phase and Reversed‐Phase HPLC methods to measure FX. Dietary supplements were purchased from local suppliers and over the internet. Samples were extracted into methanol prior to HPLC analysis. The NPLC was performed on a Diol column using a mobile phase of 6%IPA in hexane while monitoring 450nm. The RPLC was performed on a C30 column using a gradient of methanol, water, and ethyl acetate while monitoring 450nm. Samples tested contained various forms of seaweed or seaweed extracts. Products with stated FX content ranged from 250mcg to 5mg/unit. The products exhibit quite different carotenoid profiles and some do not meet label‐claims for FX. Due to current levels of obesity and increased use of thermogenic supplements, these methods will be useful to monitor the FX in current and future supplements.