HPLC Method Transfer to Narrow Bore Columns: An Evaluation

Abstract

Abstract The use of organic solvents is common in high performance liquid chromatography (HPLC). Some of these solvents, such as acetonitrile, have a very high purchase price. In addition, because of the toxic nature of these solvents, waste elimination costs have soared. The use of narrow bore HPLC columns (2mm ID or less) represents an attractive solution to the problem of excessive solvent costs by reducing the amount of HPLC mobile phase consumed. An evaluation of analysis integrity following method transfer from a standard bore column (3.9 mm ID) to a narrow bore column (2 mm ID and 1 mm ID) is necessary to determine feasibility in a QC/analytical environment.