Abstract

With the development of glycobiology and glycochemistry, bioactive polysaccharides are more and more widely used in the clinical area due to their anticoagulation, antitumor, immune regulation, antivirus, and other activities. As a marine sulfated polysaccharide, propylene glycol alginate sodium sulfate (PSS) has been commonly used as a heparinoid drug for the treatment of ischemic cerebrovascular and hyperlipidemia diseases in China for nearly 30 years. The reliable high-performance liquid chromatography (HPLC) with pre-column derivatization and post-column fluorescence derivatization methods is developed for the determination of the β-D-mannuronic acid (M)/α-Lguluronic acid (G) ratio of PSS and microanalysis of PSS in rat plasma, respectively. These methods provide the basis of the quality control, qualitative, and quantitative analysis. Especially, these methods can be used for microanalysis of other polysaccharide drugs in vivo and pharmacokinetic study. Y.-T. Xue • C.-X. Li (*) • X. Zhao • H.-S. Guan Key Laboratory of Marine Drugs, Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China e-mail: xueyiting7365676@126.com; lchunxia@ouc.edu.cn; zhaoxia@ouc.edu.cn; hsguan@ouc. edu.cn # Springer International Publishing Switzerland 2015 K.G. Ramawat, J.-M. Merillon (eds.), Polysaccharides, DOI 10.1007/978-3-319-16298-0_48 1251

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