Abstract
A new HPLC method for the analysis of bacillamide C, with DAD detector at 220nm, has been developed, validated and used in the EtOAC extracts of the fermentation broth of a Bacillus atrophaeus strain, associated with the South China Sea sponge Dysidea avara. Bacillamide C was well quantified on a reversed phase ZORBAX Eclipse XDB-C18 column by use of a gradient mobile phase from 20% (v/v) acetonitrile to 45% (v/v) acetonitrile in 15 min at a flow rate of 1 mL/min. The linearity ranges were 1.00-1000.00μg/mL with values of LOD (limit of detection) equal to 0.15μg/mL. The HPLC method was proved simple, feasible and reliable by a series of method validation experiments about linearity, accuracy, precision and stability.
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