HPLC Method Development and Validation of Chromafenozide in Paddy

Abstract

A simple and efficient HPLC-UV method was developed and validated for determination of chromafenozide in paddy as there was no previous report on record in this regard. The residue analysis method of chromafenozide, its dissipation and final residue in paddy along with soil were also studied after field treatment. Residues of chromafenozide were extracted and purified from paddy and soil followed by liquid/liquid partitioning, chromatographic column and determination by HPLC equipped with PDA detector. The separation was performed on a Phenomenex Luna RP C(18) (250 × 4.6 mm i.d, 5 μm particle size) column at room temperature. The mean accuracy of analytical method were 94.92 %, 95.38 %, 94.67 % and 96.90 % in straw, grain, soil and field water respectively. The precision (repeatability) was found in the range of 1.30 %-9.25 % for straw/grain, 1.27 %-11.19 % in soil; 1.0 %-9.25 % in field water. The precision (reproducibility) in straw/grain was ranging from 2.2 % to 12.1 %, in soil it from 2.0 % to 11.7 %. The minimum detectable concentration was 0.01 mg kg(-1). The degradation of chromafenozide formulation in rice, soil and water was determined and results showed that chromafenozide as wettable powder formulation degraded with the half-lives of about 4.4 and 2.9 days in paddy plant and soil respectively for double recommended dose. The results indicated that the developed method is easier and faster then could meet the requirements for determination of chromafenozide in paddy.