Abstract
A rapid, sensitive, and accurate high performance liquid chromatography for the determination of axitinibe (AN) in rabbit plasma is developed using crizotinibe as an internal standard (IS). Axitinibe is a tyrosine kinase inhibitor, used in the treatment of advanced kidney cancer, which works by slowing or stopping the growth of cancer cells. The chromatographic separation was performed on a Waters 2695, Kromosil (150 mm × 4.6 mm, 5 µm) column using a mobile phase containing buffer (pH 4.6) and acetonitrile in the ratio of 65:35 v/v with a flow rate of1 mL/min. The analyte and internal standard were extracted using liquid-liquid extraction with acetonitrile. The elution was detected by photo diode array detector at 320 nm.The total chromatographic runtime is 10.0 min with a retention time for axitinibe and IS of 5.685, and 3.606 min, respectively. The method was validated over a dynamic linear range of 0.002-0.2µg/mL for axitinibe with a correlation coefficient of r2 0.999.
Highlights
IntroductionPotent, and selective inhibitor of vascular endothelial growth factor receptors (VEGFR) 1, 2, and 3 (Cohenet al., 2008)
Axitinib is an oral, potent, and selective inhibitor of vascular endothelial growth factor receptors (VEGFR) 1, 2, and 3 (Cohenet al., 2008)
Chromatographic method may be used for the routine bioanalysis of axitinib
Summary
Potent, and selective inhibitor of vascular endothelial growth factor receptors (VEGFR) 1, 2, and 3 (Cohenet al., 2008). Axitinib is marketed under the name Inlyta (2014), and if one previous systemic therapy for kidney cell cancer has failed, axitinib is indicated (leaflet (Pfizer) revised 8/2014). It is soluble in DMSO (42 mg/mL at 25°C), water (
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