HPLC isolation, identification and quantification of tannins from Guiera senegalensis

Abstract

Nine galloylquinic acids, namely, 3-O-, 5-O-, 1,3-di-O-, 3,4-di-O-, 3,5-di-O-, 4,5-di-O-, 1,3,4-tri-O-, 3,4,5-tri-O- and 1,3,4,5-tetra-O-galloylquinic acid, were isolated by column chromatography and preparative HPLC from different parts of Guiera senegalensis. HPLC methods for the identification and quantification of gallotannins were perfected for the different plant parts. Isolation methods were performed using a solvent gradient dependent on the tannin composition. An isocratic method was used to quantify the main tannin (3,4,5-tri-O-galloylquinic acid) and 1,3,4,5-tetra-O-galloylquinic acid, which has already been studied with respect to its pharmacological activities, as well as 3,5-di-O- and 4,5-di-O-galloylquinic acids. The leaves, galls, stems and roots showed quantitative and qualitative differences with respect to the chemical composition of their tannins. Copyright © 2000 John Wiley & Sons, Ltd.