HPLC identification of bio active flavonoids in methanolic and aqueous leaf extracts of Schrebera swietenioides Lin.,

Abstract

Natural products continue to provide unique structural diversity in comparison to standard combinatorial chemistry, which presents opportunities for discovering mainly novel low molecular weight lead compounds. Schrebera swietenioides belonging to family Oleaceae is an important medicinal plant used in the treatment of leprosy, diabetes and hepatic disorders by ethnic people. Chromatographic methods like TLC and HPLC were used for the separation and identification of flavonoids present in methanolic and aqueous leaf extract was studied. The chromatographic methods available for the separation of flavonoids in TLC and HPLC were adopted for the study. Three compounds were identified in TLC study in methanolic and aqueous leaf extracts. In HPLC analysis, peaks corresponding to flavonoids were obtained and were identified by comparing with literature and confirm that methanolic extract contains Rutin, Quercetin and Myricetin where as in aqueous extract Rutin and Quercetin were observed. The anti diabetic activity of isolated compounds was determined by literature and confirms that compounds were found to having potent anti diabetic activity. This proves that the anti-diabetic activity of S. swietenioides was due to the presence of these bio-active compounds