Abstract
Abstract We examined with High Performance Liquid Chromatography (HPLC) gel-filtration the aggregational behaviour of insulin during application by artificial delivery systems. The short time infusion was performed by an autosyringe pump and the long time infusion was performed by a Siemens pump. For comparison we collected samples of about l ml and determined the radioimmunological activity in order to verify the results of the HPLC gel-filtration. The chromatograms, performed at 37[ddot]C and at ambibient temperature, showed no aggregates of a higher molecular weight. We observed sedimentation only once during long time infusion, however, the immediately performed gel-filtration surprisingly showed at no time an aggregate of a higher molecular weight, but the quantity of insulin was obviously reduced. A different length of the catheter systems used did not promote the aggregational behaviour.
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