Abstract
A relatively simple procedure was developed to quantitate normal and methylated tRNA bases by isocratic HPLC. tRNA was extracted with phenol from lyophilyzed cells, purified and precipitated with isopropanol. After perchloric acid hydrolysis, the samples were subjected to HPLC analysis. The mole % composition of normal and methylated bases was determined in yeasts grown on unusual carbon sources including hydrocarbons and light alcohols. Normal and methylated base composition of total tRNA depends on the microorganism and on the carbon source used.
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