Abstract
To establish an HPLC method to simultaneously determine six ingredients in zedoary turmeric oil and its related injections. HPLC analysis was performed on Waters Symmetry C18 (4.6 mm x 250 mm, 5 microm). The mobile phase was composed of methanol and water solution in gradient elution mode with at a flow rate of 1.0 mL x min(-1). The column temperature was 30 degrees C and the UV detection wavelength was 215 nm. The six ingredients were separated well. The linear ranges of curdione, curcumol, germacrone, curzerene, furanodiene and beta-elemene were 1.74-347.00, 2.38-475.00, 2.49-497.00, 2.07-826.00, 5.05-1009.00, 4.78-955.00 mg x L(-1) (r > or = 0.9996), respectively. The average recoveries were above 95% (RSD <3.0%, n=9). The method is accurate, reliable and reproducible, it can be used for the quality control of zedoary turmeric oil and its related injections.
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