Abstract
The temporal evolution of pigment composition during greening of etiolated barley leaves was investigated by reversed-phase HPLC with particular attention to chlorophyll (Chl) a′ (C132 epimer of Chl a), which had been detected by ourselves in photosystem (PS) I particles. At early stages of greening, the Chl a′/Chl a molar ratio rapidly increased to a level more than twice that in mature leaves, then gradually leveled off, accompanied by a growth of the Chl b/Chl a ratio, to the mature level. After 3 h of illumination, the temporal evolution of the Chl a′/Chl a molar ratio nearly paralleled that of the P700/Chl a ratio with a stoichiometry Chl a′/P700≅2: this strongly suggests that Chl a′ is biosynthesized as a constituent of the PS I reaction center complex.
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