Abstract
A sensitive, accurate and reproducible high performance liquid chromatographic (HPLC) procedure has been developed for the separation and analysis of lorazepam and related compounds. A 20-μL sample was separated by reversed-phase HPLC on a 150 mm ×4.6 mm, 5-μm particle, Wakosil column with 0.1m ammonium acetate (pH adjusted to 6.0 with acetic acid)-acetonitrile-methanol, 1:1:1 (v/v) as mobile phase.
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