Abstract
In this study, the concentrations of the natural hormone 17β-estradiol and the synthetic hormones levonorgestrel and 17α -ethylestradiol were determined in the water of São José do Rio Preto Municipal Dam (SP, Brazil). The SPE method was used to extract the hormones and HPLC/FLU/DAD was used to measure their concentrations. The method was validated according to Resolution-RE 899/2003 from the National Sanitary Vigilance Agency (ANVISA) for bioanalytical methods. The detection limits were 25.0, 100.0, and 50.0 ng L−1 for 17α-ethinylestradiol, 17β-estradiol, and levonorgestrel, respectively. The water samples from the dam were collected near the sewage release point and the city's water supply intake point. The sample analysis did not indicate the presence of the hormones under study, considering the detection limit of the method employed.
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