Abstract
A HPLC method was developed for the determination of gossypol in cotton seed kernel.The chromatographic separation was carried out on a ODS-C18 column (4.6 mm × 200 mm,5 μm) with a mobile phase composed of methanol and 1% phosphoric acid aqueous solution (85:15,V/V) at a flow rate of 1.0 mL/min.The detected wavelength was set at 235 nm.Results showed that a good linear relationship between HPLC peak area and gossypol concentration was obtained over the range of 1.9-34.9 μg/mL.Mean spike recoveries of gossypol at three levels were 97.8%-100.3%,with RSDs ranging from 0.4% to 1.2%.The method provides the benefits of simplicity,rapidity,sensitivity and accuracy,and therefore is applicable to the determination of gossypol in cotton seed kernel.
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