Abstract
The aim of the study was a HPLC evaluation of the lipoxygenase activity inhibiting activity of a water infusion of Ligustrum vulgare L. leaves and selected isolates from it. The antiradical activity of the water infusion was determined using DPPH, ABTS and FRAP tests. Oleuropein and echinacoside concentrations in the water infusion were determined by HPLC. Water infusion, echinacoside and oleuropein were used for an antilipoxygenase activity assay using lipoxygenase isolated from rat lung cytosol fraction. Activity of 8-LOX, 12-LOX and 15-LOX were monitored through formation of 8-HETE, 12-HETE and 15-HETE, respectively. The water infusion exhibited the highest activity against all lipoxygenases, followed by oleuropein. Echinacoside was ineffective against LOXs in lower concentrations, while higher concentration showed similar inhibition on 8-LOX and 12-LOX. 15-LOX was affected more and the presence of echinacoside remarkably decreased its activity.
Highlights
Many species of genus Ligustrum have been traditionally used worldwide in folk medicine
In previous work we have reported the inhibitory activity of a water infusion of Ligustrum vulgare
The aim of this study was an HPLC evaluation of the activity of the previously mentioned components and water infusion against LOXs isolated from rat lung cytosol fraction and their influence on different lipoxygenases (8-LOX, 12-LOX and 15-LOX)
Summary
Many species of genus Ligustrum have been traditionally used worldwide in folk medicine. In the folk medicine of Azerbaijan, the use of common privet (Ligustrum vulgare L.) (LV) leaves is linked to the treatment of hypertension [1], and is supported by recent studies of the hypotensive and diuretic effects of this plant. Chinese and Japanese medicine has used this plant due to its liver-protecting [4], antiviral [5] or anti-mutagenic effects [6]. Most of these diseases can be connected with the reactive oxygen species balance in all human tissues. In connection to the usage of privet in traditional medicine we decided to evaluate the potential anti-inflammatory activity of this plant and some its constituents. Among the different antiinflammatory test systems available, in vitro assay of inhibitory activity on lipoxygenase was chosen
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