Abstract
Iodine is an essential nutrient necessary for the production of thyroid hormones. A valuable source of iodide, which is the bio-available iodine form could be mineral waters offered by different spas. In this work, the method capable of direct determination of iodide in mineral water samples based on IAM liquid chromatography on the phosphatidylcholine column (IAM.PC.DD2 Regis HPLC) with DAD detection without sample pretreatment or any pre-concentration steps is presented. The calibration graph for iodide was linear in the range of 0.5–10.0 mg L−1 with a correlation coefficient of 0.9996. The limit of detection was 22.84 ng mL−1. The relative recoveries were in the interval of 98.5–100.2% and the repeatability, expressed as a relative standard deviation (RSD) was less than 5%. The RSA (Response Surface Analysis) investigated the effect of the sample concentration and the injection volume. The iodide concentrations in the mineral water samples ranged from 0.58 to 2.88 mg L−1. The accuracy of the method was assessed through independent analysis by ICP-MS. Iodide levels measured by these two procedures did not significantly differ. The effects of interfering ions like HCO3−, Cl−, SO42−, F−, and Br− were also tested. The analysis has shown insignificant differences in the values of the iodide peak area and its height measured in multicomponent mixtures with an error smaller than 5%.
Highlights
A commercial phosphatidylcholine column IAM.PC.DD2 Regis high performance liquid chromatography (HPLC) was applied for the quantification of iodide in mineral water samples
The obtained results agreed well with inductively coupled plasma mass spectrometer (ICP-MS) used as the reference one, which indicates good performance of both methods for iodide determination
The main advantage of the current work is the simple instrumentation covering HPLC-DAD, which is considered to be a standard in most laboratories
Summary
It is an exogenous element that can only be taken through drinking water or various foods. Iodine is absorbed by the gastrointestinal tract in the form of iodide becoming the key component of the thyroid hormones including thyroxin (T4) and triiodothyronine (T3) [1]. It is estimated that about 2 billion people in the world are affected by iodine deficiencies, including 30% of which are children [2,3]. Iodine Deficiency Disorders (IDD) cause an endemic goitre, cretinism, and are dangerous for pregnant women since they can lead to fetal brain damage and miscarriage [4,5,6]. Iodine intake for an adult has been estimated to be 1 μg per kg body weight daily [7].
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