Abstract
Carotenoids are naturally occurring fat-soluble pigments found in many organisms. Because of their extensively conjugated carbon–carbon double bond system, carotenoids are potent antioxidants. Although the most abundant carotenoid and best singlet oxygen quencher found in red tomatoes is lycopene, carotenoid profiles may vary between genotypes. The objective of this work was to perform carotenoid profile indentification using HPLC-DAD-APCI-MS in ten wild cherry tomato accessions and one cultivated tomato. A mixture of hexane/acetone/ethanol (50:25:25) and 0.1% BHT was used for carotenoid extraction. For separation, a C30 column at 30 °C with a gradient consisting of methanol, methyl-tert-butyl ether, and water was used for their analysis. Ten major carotenoids were quantified within cherry tomato samples. All accessions present different profiles and quantities of carotenoids. Wild red tomatoes had more lycopene content that commercial tomato, whereas yellow tomatoes present no lycopene. From a functional viewpoint, higher concentrations of carotenoids that could play an antioxidant activity were measured from accessions IAC401, IAC426, LA1480, IAC391, and LA2692. This trait means that these germplasms may be targets for commercial activities. To the best of our knowledge, this is the first time that HPLC-DAD-APCI-MS has been used to analyze these accessions of wild cherry tomatoes that are both functionally promising and suitable for projects with social implementation at a local scale.
Highlights
Carotenoids are naturally occurring fat-soluble pigments found in many organisms
UV-Vis absorption properties, and taking into account the diversity and concentration of carotenoids within wild tomatoes compared to commercial tomatoes, this study aimed to analyze the carotenoid profile of ten wild cherry tomato accessions and one cultivated tomato using high-performance liquid chromatography (HPLC) coupled with atmospheric pressure chemical ionization (APCI) interphase and diode array (DAD) and mass spectrometry (MS) as a specific platform for carotenoid analysis
The proposed method based on liquid chromatography coupled with diode array and mass spectrometry detection (HPLC-diode-array detector (DAD)-MS) allowed the identification and quantification of carotenoids in cherry tomatoes
Summary
Carotenoids are naturally occurring fat-soluble pigments found in many organisms. Because of their extensively conjugated carbon–carbon double bond system, carotenoids are potent antioxidants. Higher concentrations of carotenoids that could play an antioxidant activity were measured from accessions IAC401, IAC426, LA1480, IAC391, and LA2692 This trait means that these germplasms may be targets for commercial activities. 1. Introduction with regard to jurisdictional claims in Carotenoids are the second most abundant naturally occurring pigments on earth, with more than 750 members ranging from colorless to yellow, orange, and red colors, with variations reflected in many fruits, flowers, and vegetables [1]. Introduction with regard to jurisdictional claims in Carotenoids are the second most abundant naturally occurring pigments on earth, with more than 750 members ranging from colorless to yellow, orange, and red colors, with variations reflected in many fruits, flowers, and vegetables [1] These pigments, mainly composed of C40 lipophilic isoprenoids, protect cells from reactive radicals and prevent lipid peroxidation [2]. The most important carotenoids for humans include published maps and institutional affiliations
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