HPLC/DAD Analysis and Antioxidant Activity of Adlay Sprouts and Seeds

Abstract

Adlay is an annual plant known for its abundant bioactive compounds and diverse pharmacological activities. Coixol, a key component found in various parts of adlay, significantly contributes to its biological activity. This study was conducted to extract adlay sprouts and seeds using different solvents (methanol and ethanol) and extraction methods (reflux and ultrasonic extraction). The extracts were then evaluated for their total polyphenol and flavonoid contents, as well as antioxidant ability (DPPH and ABTS+). Additionally, the coixol content of these extracts was analyzed using HPLC/DAD analysis. The results showed that the extraction methods and solvents used impacted the bioactive compounds and their activities in the samples. Adlay sprouts exhibited a higher compound content and stronger antioxidant capacity than adlay seeds. Moreover, a substantial amount of coixol was found in the sprouts, while it was not detected in the seeds. This study emphasizes the importance of selecting appropriate extraction methods to optimize the biological activities of adlay sprouts and seeds. Adlay sprouts, with their enriched phytochemical compounds and enhanced antioxidant ability, could serve as a valuable material for health product applications.