Abstract
Тhe aim of the study was identification and determination of the content of flavonoids in dry extracts of Teucrium polium, collected from Republic of Macedonia. Two different drying procedures were used, freeze and spray draying. In freeze-dried (FDE) and spray-dried (SDE) extracts of aerial parts of T. polium five flavone aglycones (luteolin, apigenin, cirsiliol, cirsimaritin and cirsilineol) were identified by HPLC method, on the base of retention times and UV spectral data of the components of the extracts in comparison to the authentic samples of flavonoids. Additionally, seven glycosides of apigenin and luteolin were tentatively identified. No significant differences in the composition nor in the content of identified flavonoids were found between both extracts. The content of total flavonoids determined by UV-spectrophotometry with AlCl3 was 72.8 ± 0.62 mg Lut/g and 73.34 ± 0.53 mg Lut/g in FDE and SDE extract of T. polium, respectively.
Highlights
Teucrium polium L. (Lamiaceae) is widely distributed wild-growing plant in Republic of Macedonia (RM) (Micevski, 1998) and abounds in southwestern Asia, Europe, and North Africa (Mashreghi and Niknia, 2012)
There is a presence of different classes of secondary metabolites, such as diterpenes (Malakov and Papanov, 1983; Marquez and Valverde, 1979), essential oil (Cozzani et al, 2005; Moghtader, 2009; Kabouche et al, 2007), phenylethanoid glycosides (Oganesyan et al.,1991), etc, probably the most important group of compounds in T. polium are the flavonoids
The HPLC analysis of dissolved dry extract in methanol (1 mg ml-1) and comparison of the retention times and UV spectral data of selected picks with the appropriate data obtained for standard substances showed presence of 5 different flavones in both dried extracts of T. polium and seven glycosides of apigenin and luteolin (Table 1)
Summary
Teucrium polium L. (Lamiaceae) is widely distributed wild-growing plant in Republic of Macedonia (RM) (Micevski, 1998) and abounds in southwestern Asia, Europe, and North Africa (Mashreghi and Niknia, 2012). (Lamiaceae) is widely distributed wild-growing plant in Republic of Macedonia (RM) (Micevski, 1998) and abounds in southwestern Asia, Europe, and North Africa (Mashreghi and Niknia, 2012) This flowering perenial small shrub can grow up to 30 cm and has a callous white exterior. Spray-dried extracts are suitable for production of cold water soluble tea and herbal extracts, hot water soluble tea, industrial use for the food and beverage industries, dry sugar mixes, “Ready to Drink” tea beverages, various food products, and tea powder blends like “2 in 1” or “3 in 1” milk mixes. Freeze dried extracts are mainly hot water soluble, suitable for consumer packs of high value products
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