Abstract
Skin secretory amphibian antimicrobial peptides are the part of their immune defense. The present work is devoted to the study of the influence of “water environment stress” and additional bacterial impact on the composition of the skin secretion of the Common frog (Rana temporaria) by means of high-performance liquid chromatography (HPLC) and matrix assisted laser desorption/ionization (MALDI) mass spectrometry. It was shown that the contact of the amphibian species with Micrococcus luteus and Staphylococcus aureus stimulates the release of antimicrobial peptides, maintains the high bradykinin and related peptides levels in the skin secretion and influences the processing of the latter ones. The possibilities of mass spectrometric profiling by using HPLC and MALDI were demonstrated. This feature allows the detection of potentially bioactive peptides for their future direct testing, as has been shown for temporin M and brevinin 1Tb in the present study.
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