Abstract
A high performance liquid chromatographic method has been developed for the separation and quantitation of sucrose ester analogs using an evaporative light scattering detector (ELSD). By varying the mobile phases and gradient conditions, the complex mixtures of synthetic or commercial sucrose esters were completely separated into several fractions, such as mono‐, di‐ or higher esters, with different regioisomers in each one on a single run. A separation and quantitative method of ten pure monoesters and diesters was also described to evaluate the method's potential application in the quantitation of all sucrose ester analogs using external standards. The described method is found to be accurate, relatively inexpensive, straight forward, and reproducible, and in principle can be readily applied to the analysis of any sucrose ester analogs of interest with little variation, to meet all requirements of qualitative and quantitative analytical procedures.
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