Abstract
A two-solvent system was used in high-performance liquid chromatography (HPLC) analysis of radiolabeled pharmaceutically active proteins. The proteins were successively labeled with [67Ga]-gallium chloride separately after conjugation with diethylenetriaminepentaacetic acid dianhydride (ccDTPA). The radiolabelded proteins were purified using size exclusion chromatography and/or solid-phase purification. A two-solvent system consisting sodium acetate/citrate buffer was used for the determination of radiochemical purity (90–98%), specific activity (22–23 TeBq/mM), and labeling efficiency (70–82%) at optimized conditions in each case. The retention times for insulin, β-HCG, gonadotropin, erythropoietin, glucagon, (1-24-corticotrophin, rituximab, oxytocine, and streptokinase were 17.2, 14.8, 18.31, 12.77, 20.0, 21.97, 15.2, and 17.2 min, respectively, while for 67Ga3+ cation and/or 67Ga-DTPA were at the range of 2.70–2.90 min. This is a simple and inexpensive HPLC method for the analysis of radiolabeled peptides and proteins using reverse-phase columns.
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