Abstract
The antioxidant activities of crude extract, dichloromethane, ethyl acetate, and butanolic fractions of Nasturtium officinale were investigated using the 1,1,-diphenyl-2-picryl-hydrazyl and thiobarbitaric acid reactive species methods. Total phenolics and flavonoids were determined. Rutin, chlorogenic, and caffeic acids were quantified in the crude extract by high performance liquid chromatography-photodiode array detector. The antioxidant and radical scavenging activity of the crude extract and fractions were found in the following decreasing order: butanolic fraction > ethyl acetate fraction > dichloromethane fraction > crude extract. The extent of antioxidant activities was in accordance with the amounts of phenolics and flavonoids present in the extract and fractions. Ethyl acetate and butanolic fractions caused a sharp fall in thiobarbitaric acid reactive species production, starting at the concentration of 1 μg/mL for both fractions with IC50 6.92 ± 0.19 and 12.07 ± 0.45 μg/mL, respectively. N. officinale might be a valuable antioxidant natural source and seemed to be applicable in both healthy medicine and the food industry.
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