Abstract
A simple and effective HPLC assay for determination of the main phenolic acids (rosmarinic, caffeic, protocatechuic) that were isolated from medicinal plant Melissa officinalis has been developed. The main goal of this work was to test and evaluate the liquid extraction procedure for plant samples. As the effective extraction media, mixtures of methanol-water and methanol-water (pH 2.5) were chosen. The following extraction conditions were used: time of extraction step 10 minutes, 3 extraction steps, volume of extraction mixture 10 mL, temperature 25°C. The extraction recoveries were: rosmarinic acid 100.7%, caffeic acid 99.9%, protocatechuic acid 96.0%. Yields of phenolic acids extracted from Melissa officinalis grown in Slovakia were: rosmarinic acid 17.03 mg/g (RSD=1.87%), caffeic acid 1.99 mg/g (RSD=3.65%), and protocatechuic acid 0.041 mg/g (RSD=5.75%).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
