Abstract
A simple and effective HPLC assay for determination of the main phenolic acids (rosmarinic, caffeic, protocatechuic) that were isolated from medicinal plant Melissa officinalis has been developed. The main goal of this work was to test and evaluate the liquid extraction procedure for plant samples. As the effective extraction media, mixtures of methanol-water and methanol-water (pH 2.5) were chosen. The following extraction conditions were used: time of extraction step 10 minutes, 3 extraction steps, volume of extraction mixture 10 mL, temperature 25°C. The extraction recoveries were: rosmarinic acid 100.7%, caffeic acid 99.9%, protocatechuic acid 96.0%. Yields of phenolic acids extracted from Melissa officinalis grown in Slovakia were: rosmarinic acid 17.03 mg/g (RSD=1.87%), caffeic acid 1.99 mg/g (RSD=3.65%), and protocatechuic acid 0.041 mg/g (RSD=5.75%).
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
