Abstract
Reversed-phase high-performance liquid chromatography (RP-HPLC) method with C 4 column and C 18 column for analyzing β-lactoglobulin and α-lactalbumin in bovine milk was developed and the performance and characteristic of two columns were compared. Shiseido Proteonavi C 4 column (250 mm×4.6 mm×5 μm) and Shiseido CAPCELL PAK SG 300 C 18 column (250 mm×4.6 mm×5 μm) were used in the experiment. Phase A was composed of 0.1% (V/V) trifluoroacetic acid (TFA) in ultrapure water and Phase B (organic phase) was composed of 0.1% TFA in acetonitrile. Gradient elution was taken. Flow rate was 1 mL min −1 . The detection wavelength was 215 nm. The injection volume was 20 μL and the column temperature was 30°C. The results showed that linear relationship was good and recovery of α-lactalbumin and β-lactoglobulin was 86.12%–104.38%, C 18 column had stronger ability to resist acid and more stable, and the method with C 4 column had excellent sensitivities and good separation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Northeast Agricultural University (English Edition)
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
