Abstract
Abstract A simple and rapid HPLC method was developed to quantitate the in vitro release of estrone from poly(l-lactic acid) microspheres. Propylparaben was used as the internal standard. The method employed a reversed-phase column (LiChrosorb 5μm, RP-18, 125 × 4 mm I.D.). The isocratic mobile phase system was a 60:40 (v/v) mixture of methanol and aqueous acetate buffer (pH 5.4). Standard curves were linear over the desired range at both 214 nm and 280 nm. Because of the greater sensitivity at 214 nm this wavelength was chosen for further analyses. A flow rate of 1.8 ml/min yielded a retention time of 3.5 minutes for estrone and 2.0 minutes for the internal standard and the method was found to be reproducible (RSD of less than 3%). The molar absorpuvities for estrone in different solvents and the mobile phase are presented.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
