Abstract
An isocratic, reverse phase HPLC method, for the simultaneous determination of 5-fluorouracil (5-Fu), 5-fluoro-5, 6-dihydrouracil (FuDH), and 5-fluorouridine (Furd), in rat plasma has been developed. The method utilizes a Spherisorb® column and a mobile phase composition of 100 μM phosphate buffer at pH 3.0. The extraction procedure from the plasma samples has been optimized to give maximum recovery of the analytes. The method has been validated as per USP guidelines in terms of linearity, accuracy and precision. The limit of quantitation for 5-Fu, FuDH, and Furd was found to be 0.2 μg/mL, 0.3 μg/mL and 0.8 μg/mL respectively.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
