HPLC Analysis and Antioxidant Properties of Algerian Lepidium draba Ethyl acetate Extract

Abstract

The aim of this study was to identify and quantify the phenolic compounds of the Ethyl acetate extract from Lepidium draba using HPLC-TOF/MS; and to investigate the antioxidant potencies. In this work, we have found that the Ethyl acetate soluble fraction of L. draba is a good source of antioxidant, which is the result of its phenolic acids and flavonoids. Five methods were conducted, to determine the antioxidant components of the Ethyl acetate extract: DPPH, ABTS, CUPRAC, Reducing power assay and Metal chelating assay. Nineteen compounds from which 13 phenolic acids and 6 flavonoids known for their pharmacological properties were identified by using HPLC-TOF/MS technique: Gallic acid, Gentisic acid, Chlorogenic acid, 4-Hydroxy benzoic acid, Protocatechuic acid, Caffeic acid, Vanillic acid, 4-hydroxybenzaldehyde, p-coumaric acid, Chicoric acid, Ferulic acid, Rosmarinic acid, Salicylic acid, Catechin, Rutin, Hesperidin, Apigenin-7-glucoside, Quercetin, and Kaempferol. All These compounds were identified and quantified in Lepidium draba for the first time. The IC50 and A0.50 of the Ethyl acetate extract were 305.69±1.22, 39.42±0.45, 152.27±0.38 and 183.83±2.47 μg/mL in DPPH, ABTS, Metal chelating assay, and CUPRAC. However, the results indicate that L. draba is a good source of natural antioxidants.