Abstract
Oral phosphodiesterase-5 (PDE5) inhibitors and intracavernous injection of prostaglandin E1 (PGE1) are treatments of choice for post radical prostatectomy (RP) erectile dysfunction (ED). However, these treatments are not necessarily effective and the development of a novel and effective therapy for post RP ED is required. Recently, it was reported that mesenchymal stem cell (MSC) transplantation induces nerve regeneration and improves erectile function in a rat model of bilateral cavernous nerve injury (BCNI). However, unexpected differentiation of the transplanted stem cells is still an issue with this approach. To avert this problem, we explored the suitability of filtrated stem cell lysate (FSCL), which contains secreted factors but does not contain MSCs. In this study, we investigated the effects of FSCL on erectile function in a rat model of BCNI. Eight-week-old male Wistar/ST rats underwent BCNI or sham surgery. After surgery, filtrated adipose-derived stem cell lysate (FADSCL) or filtrated bone marrow-derived stem cell lysate (FBMSCL) was injected into the corpus cavernosum. After 4 weeks, erectile function was evaluated by measuring intracavernosal pressure/mean arterial pressure under stimulation of the cavernous nerve. Proteomic analysis was performed to identify effective factors in FSCL.
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