HP-01-004 The effects of antifibrotic agent-pirfenidone on penile cavernous function in experimental ischaemic priapism-induced rat model

Abstract

Aim of the study was to assess the impact of an antifibrotic and anti inflammatory agent-pirfenidone in an experimental ischemic rat priapism model. 0Forty-eight male Albino-Wistar rats were randomized into four groups (n=12 in each group): Group-1 (non-priapism, no-medication), Group-2 (one-hour priapism, no-medication), Group-3 (one-hour priapism, six-weeks followup, no-medication), and Group-4 (one-hour priapism, six-weeks pirfenidone). Erection model was created by electrical stimulation of the cavernous nerve. The intracavernosal pressure (ICP) and the blood pressure were recorded (Fig. 1a). Degree of the fibrosis were evaluated as (+), (++), (+++), and (++++) using Masson's trichrome strain. Malondialdehyde (MDA) and reducted glutathione (GSH) levels were measured by spectrophotometric method. Statistical analyses were performed with SPSS program for windows. Group-2 and 3 showed marked cavernosal fibrosis following the formation of an ischemic priapism model. In contrast, Group-4 showed significant decrease in fibrosis compared to Group-3 (Fig. 1b). The mean basal ICP (BICP), maximum ICP (MICP), mean ICP (MeICP), the difference between MICP and BICP (DICP), area under curve (AUC), and the ratio of MeICP/ mean intraarterial blood pressure (MIBP) were recorded. In Group-2, all these parameters showed significant decrease compared to Group-1 (p<0.05). The mean MICP, MeICP and the MeICP/MIBP ratios significantly decreased in Group-3 compared to Group-1 (p<0.05). The mean BICP, MICP, MeICP, DICP, AUC, and MeICP/MIBP values were similar in Group-1 and 4 (p>0.05) (Fig. 1c). Group-4 showed a statistically significant decrease in cavernosal mean MDA levels compared to Group-3, (p<0.05). Group 4 showed a statistically significant increase in the mean GSH levels compared to Group-3 (p<0.05) (Fig. 1d).