Abstract
BackgroundHOXA cluster antisense RNA2 (HOXA-AS2), a long-chain non-coding RNA, plays an important role in the behavior of various malignant tumors. The roles of HOXA-AS2 in endometrial cancer remain unclear.MethodsWe test expression levels of HOXA-AS2, miRNA-302c-3p, the transcription factor zinc finger X-chromosomal protein (ZFX), and the chitinase-like protein YKL-40 in endometrial carcinoma by qRT-PCR and western blotting. Luciferase reporter and qRT-PCR assays were conducted to identify potential binding sites of HOXA-AS2 to miRNA-302c-3p. Cell cycle, migration and invasion ability of endometrial cancer cells were investigated using flow-cytometric analysis, CCK-8 and transwell assays, respectively.ResultsHOXA-AS2 levels were significantly increased in endometrial cancer specimens compared to normal endometrial specimens. Upregulated HOXA-AS2 promoted invasion and proliferation of type I endometrial cancer cells. HOXA-AS2 silenced miRNA-302c-3p by binding to it. MiRNA-302c-3p negatively regulates ZFX and YKL-40. Thus HOXA-AS2 promotes the development of type I endometrial cancer via miRNA-302c-3p-mediated regulation of ZFX.ConclusionsThese findings suggest that HOXA-AS2 can act as a new therapeutic target for type I endometrial cancer.
Highlights
HOXA cluster antisense RNA2 (HOXA-AS2), a long-chain non-coding RNA, plays an important role in the behavior of various malignant tumors
We discovered that HOXA-AS2 was located in the cytoplasm (Additional file 3: Fig. S1a)
Using qRT-PCR and western blotting, we found that zinc finger X-chromosomal protein (ZFX) and YKL-40 were significantly higher in endometrial cancer tissues than in normal endometrial tissues both mRNA and protein levels (Fig. 4b, c)
Summary
HOXA cluster antisense RNA2 (HOXA-AS2), a long-chain non-coding RNA, plays an important role in the behavior of various malignant tumors. The roles of HOXA-AS2 in endometrial cancer remain unclear. Chemotherapeutic and bioimmunotherapeutic tumor treatments, cancer remains difficult to treat. Endometrial carcinoma is one of the most common gynecological malignancies. 380,000 new cases of endometrial cancer occured and 89,000 cases died in. LncRNAs regulate gene expression at multiple levels, including transcriptional and posttranscriptional levels [3]. They play important roles in the diagnosis, treatment, and prognosis of tumors [4, 5]. Roles of some lncRNAs in endometrial carcinoma have already been clarified.
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