Abstract
The HOX homeodomain is a large family of transcription factors involved in gene regulation, both as transcriptional activators and repressors, at many stages in development. Interactions with other proteins, such as PBC homeodomain proteins, can increase HOX protein affinity and specificity for DNA binding. Shen et al . show that HOX proteins also interact with CBP, a transcriptional regulator with histone acetyltransferase activity. Different members of the HOX family interacted with CBP through the NH 2 -terminus and the homeodomain, which has a putative consensus acetylation motif. However, the HOX proteins were not acetylated by CBP, instead they inhibited CBP HAT activity in vitro and in vivo. In vivo, this inhibition blocked CBP-mediated increases in reporter gene expression. Addition of CBP to HOX, PBC, and DNA complexes promoted the disassociation of the HOX from DNA based on electrophoretic mobility gel shift assays. Shen et al. suggest that the repressive effects of HOX proteins may be the result of inhibition of CBP HAT activity. W.-f. Shen, K. Krishnan, H. J. Lawrence, C. Largman, The HOX homeodomain proteins block CBP histone acetyltransferase activity. Mol. Cell. Biol. 21 , 7509-7522 (2001). [Abstract] [Full Text]
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
