HOX Antisense Intergenic RNA Inhibitor Loaded on Magnetic Nanoparticles Inhibits Gastric Cancer Progression Through miR-20a-5p

Abstract

Magnetic nanoparticles are easy to operate and highly efficient and acts as ideal molecular carriers. At the same time, research has found that inhibiting Homeotic Complex Gene transcript antisense RNA (HOTAIR) can inhibit tumor development. Therefore, this study intends to use magnetic nanoparticles to carry HOTAIR inhibitors to explore its role in gastric cancer progression. Magnetic nanoparticles loaded with HOTAIR inhibitor and mouse model of gastric cancer were constructed. Serum HOTAIR level was detected by ELISA, tumor volume and mass changes in mice were observed, and histopathology was assessed by HE staining. The effect of HOTAIR inhibitor loaded magnetic nanoparticles on miR-20a-5p expression and downstream apoptosis genes/proteins was also evaluated. (1) si-HOTAIR is the silent gene of HOTAIR. The obtained si-HOTAIR magnetic nanoparticles were used in various research groups. It was found that HOTAIR was highly expressed in gastric cancer mice. (2) HOTAIR inhibitor intervention reduced tumor mass and volume, and changed gastric histopathological conditions. (3) HOTAIR inhibitors inhibited the activities of gastric cancer MFC cells, promoted apoptosis, and downregulated miR-20a-5p. (4) Compared with other groups, miR-20a-5p in the cells of si-HOTAIR-loaded magnetic nanoparticles group was reduced and cancer progression was significantly inhibited. Magnetic nanoparticles loaded with HOTAIR inhibitors have a strong anti-tumor effect and this effect is achieved by inhibiting miR-20a-5p. At the same time, this process is related to Bcl-2.