Abstract
The dynamics of molecules in solution is usually quantified by the determination of timescale-specific amplitudes of motions. High-resolution nuclear magnetic resonance (NMR) relaxometry experiments—where the sample is transferred to low fields for longitudinal (T1) relaxation, and back to high field for detection with residue-specific resolution—seeks to increase the ability to distinguish the contributions from motion on timescales slower than a few nanoseconds. However, tumbling of a molecule in solution masks some of these motions. Therefore, we investigate to what extent relaxometry improves timescale resolution, using the “detector” analysis of dynamics. Here, we demonstrate improvements in the characterization of internal dynamics of methyl-bearing side chains by carbon-13 relaxometry in the small protein ubiquitin. We show that relaxometry data leads to better information about nanosecond motions as compared to high-field relaxation data only. Our calculations show that gains from relaxometry are greater with increasing correlation time of rotational diffusion.
Highlights
Understanding the molecular determinants of protein function requires the characterization of their structural properties and of their dynamics
We use the detector approach to dynamics, which we have recently introduced in the context of solid-state nuclear magnetic resonance (NMR) and later adapted to solution-state NMR (Smith et al 2018, 2019a, b), to identify the ranges of correlation times probed by ensembles of relaxation rate constants collected with high-resolution relaxometry or high-field NMR alone
High-resolution relaxometry provides information about motion with longer correlation times, which cannot be properly described with the lower resolution available from high-field relaxation alone
Summary
Understanding the molecular determinants of protein function requires the characterization of their structural properties and of their dynamics. The sensitivities obtained for a set of detectors depends on the sensitivity of the experimental rate constants to internal motion, and this in turn depends on the correlation time of the molecular tumbling.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
