Abstract
Drug delivery systems can be engineered to enhance the localization of therapeutics in specific tissues in response to externally applied stimuli and/or local environmental changes. In recent decades, efforts to improve drug delivery techniques at both nano- and macroscale have led to a new era of therapeutic efficacy. Such technological advancements resulted in improved drug delivery systems regularly entering the clinical setting. However, these delivery innovations are unfortunately not always readily applied to newly developed technologies. One of these new and exciting technologies that has been overlooked by drug delivery scientists is prime editing. Prime editing is a novel genome editing technology that exhibits the plug-and-play capability of CRISPR/Cas9 editors while avoiding double-strand DNA breaks throughout the entire process. This article focuses on describing the potential advantages and disadvantages of selecting nanomedicine technologies along with prime editing capabilities for the delivery of cargo.
Highlights
Prime editing technologies have the potential to essentially revolutionize current genome-editing practices
CRISPR/Cas9 systems utilize either nonhomologous end-joining or homology-directed repair to restore DNA viability, both of which involve the repair of double-stranded DNA breaks [3]
What Is Prime Editing? Prime editing is a versatile genome editing method that “writes” new genetic information into precise DNA locations. is method differs from CRISPR/Cas9 systems in that it employs a catalytically impaired Cas9 endonuclease fused to a reverse transcriptase that is programmed via prime editing guide RNA [1]. pegRNA encodes both the desired edit and the target DNA site. e prime editing method enables DNA insertions, deletions, and substitutions without requiring doublestrand DNA breaks or exogenous donor DNA templates
Summary
Prime editing technologies have the potential to essentially revolutionize current genome-editing practices. Prime editing serves to overcome many of the limitations of the current CRISPR systems by allowing for genome. Genetics Research manipulation using only single-strand DNA breaks (Figure 1) and may potentially give rise to a new frontier in genome editing research. Prime editing is a versatile genome editing method that “writes” new genetic information into precise DNA locations. E prime editing method enables DNA insertions, deletions, and substitutions without requiring doublestrand DNA breaks or exogenous donor DNA templates. By avoiding the sporadic DNA repair associated with doublestrand breaks, prime editing can improve the accuracy of gene editing in vitro and theoretically in vivo. To achieve widespread in vivo use, effective delivery of the prime editing machinery is required. The development of effective nonviral delivery systems for prime editors will likely require innovation and a thorough evaluation of delivery system requirements
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